The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 ?g high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.
See here or here for the handbook for the Qiagen Spin Miniprep Kit. If you have never done this protocol before, read the the background information in the handbook (like the Important Notes section). It contains useful information. The following has been reproduced from the handbook and annotated based on experience with the kit.
QIAprep Miniprep Handbook 05/2012 7 Introduction The QIAprep Miniprep system provides a fast, simple, and cost-effective plasmid miniprep method for routine molecular biology laboratory applications. QIAprep Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.
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Q I A p r e p S p i n QIAprep Miniprep Handbook 05/2012 19 Protocol: Plasmid DNA Purification using the QIAprep Spin Miniprep Kit and a Microcentrifuge This protocol is designed for purification of up to 20 µg of high-copy plasmid DNA from
QIAprep Spin Miniprep Kit Protocol. 1-5 ml overnight cultures of E.coli in LB (Luria-Bertani) medium. (Spin bacterial cells at 500 x g for 10 minutes. (Resuspend pelleted bacterial cells in 250 (l of Buffer P1 and transfer to a microfuge tube. (Add 250 (l of Buffer P2 and gently invert the tube 4-6 times to mix.
QIAprep Miniprep Handbook 06/2005 7 Storage QIAprep Miniprep Kits should be stored dry at room temperature (15-25°C). Kits can be stored for up to 12 months without showing any reduction in performance and qual ity. For longer storage these kits can be kept at 2-8°C. If any precipitate forms in the buffers
Qiagen, Inc. QIAPREP SPIN MINIPREP KIT (250 QIAPREP SPIN MINIPREP KIT QIAprep Spin Miniprep; Qiagen; For 1-24 high-purity plasmid minip
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